Fig. 6
Analysis of sex-dependent differences in life span and motor-behavioral outcomes of AAV9-treated Cln8mnd mice. AAV9-treated Cln8mnd mice live similar lifespans regardless of sex (A). AAV9-treated Cln8mnd mice perform similarly in an accelerated rotarod test (B). Transient sex-dependent differences detected at 8 and 18 months of age in the pole climb measurement “time to descend pole” (C), while no differences were detected in “time to turn down pole” (D), and differences detected at 10 and 12 months of age in “number of falls from the pole” (E). No differences detected in tremor presence between the sexes of AAV9-treated Cln8mnd mice (F-I) Cln8mnd females treated with AAV9-CLN8 were significantly slower at the Morris water maze (MWM) at 6 and 8 months of age (J), which was not accounted for by swim speed (K). No differences were observed in completion time of the reverse MWM (L) but AAV9-treated Cln8mnd females had a higher swim velocity (M). Survival curve: log-rank (Mantel–Cox); n = 15–16 animals/sex. Two-way ANOVA with Fisher’s LSD post-hoc. Mean ± SEM, n = 5–11 animals/sex/group, detailed n described in Additional file 4: Table S1. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001