Fig. 3
Sex dependent differences in Cln8mnd life span and motor-behavioral assays. Cln8mnd females have a decreased life span compared to Cln8mnd males, with a median age of 9.5 months and 10 months respectively (A). Morris Water Maze (MWM) demonstrating Cln8mnd females taking significantly longer to complete the task at 2 and 6 months of age when compared to Cln8mnd males (B), of which was not accounted for by differing swim speed (C). Cln8mnd females completed the reverse MWM in greater time compared to their male counterparts despite no difference in swim speeds (D, E). Comparisons of wild type males versus wild type females^, Cln8mnd males versus Cln8mnd females*, Cln8mnd males versus wild type males#, and Cln8mnd females versus wild type females#. Survival curve: log-rank (Mantel–Cox); n = 13–16 animals/sex. MWM: Two-way ANOVA with Fisher’s LSD post-hoc. Mean ± SEM, n = 2–11 animals/sex/group, detailed n described in Additional file 4: Table S1. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001