Fig. 2
Female Cln8mnd mice show enhanced glial activation in brain. Male Cln8mnd mice demonstrate greater astrocyte expression (GFAP+) within the VPM/VPL of the thalamus at 6 months of age (A) whereas female Cln8mnd mice have greater expression within the S1BF at 4 and 8 months of age (B). Female Cln8mnd mice exhibit enhanced microglial activation (CD68+) at months 4, 6, and 8 within the VPM/VPL (C) and at month 4 within the S1BF (D). Two-way ANOVA with Fisher’s LSD post-hoc. Mean ± SEM, n = 1–4 animals/sex/group, detailed n described in Additional file 4: Table S1. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001. Scale Bars: 150 µm