Table 1 Optimized gradient for LC-MS/MS analysis for the quantification of cysteamine in plasma.
Time | % Eluent A | % Eluent B | Flow |
|---|---|---|---|
0.0 | 99 | 1 | 0.4 mL/min |
6.0 | 80 | 20 | 0.4 mL/min |
6.5 | 0 | 100 | 0.4 mL/min |
8.5 | 0 | 100 | 0.4 mL/min |
8.6 | 99 | 1 | 0.4 mL/min |
14 | 99 | 1 | 0.4 mL/min |