Fig. 6

Identification of target genes regulated by Activin-A in FOP-ML. A and B, Venn diagrams showing the overlap of DEGs between different comparison groups for up-regulated (A) or down-regulated (B) genes. C and D, The list of up- (C) and down-regulated (D) genes. E and F, Effect of TGFβ and BMP signal inhibitors on the expression of ID3 (E) or LYVE-1 (F) genes in Activin-A treated FOP-ML. Cells were treated simultaneously with Activin-A (100 ng/mL) and SB431542 (SB) (5 µM) or DMH1 (5 µM). RNAs were extracted at each time point and assessed by quantitative reverse transcriptase PCR (qPCR). The expression levels were normalized to those of FOP-ML before treatment. The samples were collected from three biologically independent experiments. Two-way ANOVA followed by Dunnett's multiple comparison test (vs. DMSO group). *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001