Fig. 2

Characteristics of FOP- and resFOP-MLs with or without stimulations. FOP- and resFOP-MLs were treated with either LPS (10 ng/mL) or Activin-A (100 ng/mL) for 24 h. A, Immunostaining of CD14, CD16, and p-Smad5. Cells were stained before or after 24 h treatment of each chemical. Scale bar = 50 μm. B–E, Time course analyses of mRNA expressions aftere stimulation with LPS or Activin-A. RNAs were extracted at each time point and assessed for the expression of CD14 (B), CD16 (C), INHBA (D), and FOP-ACVR1A (E) by qPCR. The expression levels was shown as a value relative to those of resFOP-ML before treatment. The results were obtained from four biologically independent experiments. The error bars indicate standard deviation. Tukey–Kramer test *p < 0.05, **p < 0.01