Fig. 4

Electrophysiological measurements of N856H Kv10.2 channel expressed in COS7 cells, in presence or absence of AZA or RTG drugs. A Original current recordings of N856H Kv10.2 channels in the absence or presence of AZA, as indicated, after applying the pulse protocol shown on the top. Current traces are shown in 20-mV increments for clarity. B Current–voltage relationship obtained after plotting the amplitude of the current measured at the end of the 3-s pulses versus membrane potential in the absence and in the presence of AZA. C Time constant of activation of N856H Kv10.2 mutant channels at + 50 mV cells in the absence or in the presence of AZA. D–F As in A–C but with RTG instead of AZA. G For comparison, WT (wild type protein) Kv10.2 currents with the exponential fit of activation. H Current recordings elicited after the activation of N856H Kv10.2 mutant channels in the absence or presence of AZA. I Same as in H but with RTG instead of AZA. Data are expressed as the mean ± SEM of n = 4 (for AZA) and n = 5 (for RTG). *p < 0.05, ***p < 0.001