Fig. 2From: Early embryonic lethality in complex I associated p.L104P Nubpl mutant miceExpression of Nubpl p. L104P allele by cDNA sequencing. A Relatively similar levels of wild type and p. L104P is expressed. B PCR primers span multiple exons and exon splice junctions were correctly confirmed. Silent mutations downstream of p. L104P mutation were added to inhibit cutting by Cas9 Crispr during mouse model development. C, D Nubpl mouse: human amino acid homology. Known disease associated aa mutations are shown in redBack to article page