Fig. 1

Targeted validation of exodosis-related proteins. A MANF HTRF assay of media collected from T0034 skeletal muscle cell line treated with 100 nM Tg for 8 h. Extracellular MANF is increased following Tg treatment, mean ± SEM, n = 6 wells/treatment group, ***p = 0.0008, unpaired two-tailed t-test. B Carboxylesterase 1/2 fluorescence activity measured in media collected from T0034 skeletal muscle cell line treated with 100 nM Tg for 8 and 24 h, mean ± SEM, n = 10/treatment group, ****p < 0.0001, 2-way ANOVA, Sidak’s multiple comparison test. C PDIA1/P4HB IP of media collected from T0034 skeletal muscle cell line treated with 100 nM Tg for 8 h, mean ± SEM, n = 4/treatment group, *p = 0.022, unpaired two-tailed t-test. Of note, bands at approximate 28 kDa and 51 kDa are IgG light and heavy chains from IP, respectively