Table 2 The primers for the Sanger sequence
Genes | Variant detection | Forward primer | Backward primer |
|---|---|---|---|
LMNA | Chr1:156104681 | 5′AATTCTGATTTTGGTTTCTGT3′ | 5′GGGTTAGGACTGTGGTGAC3′ |
ACTA1 | chr1:229568310 | 5′GTCGGTATGGGTCAGAAAGAT3′ | 5′ATGAAGGAGGGCTGGAAGA3′ |
RYR2 | chr1:237666765 | 5′TGACCAGTTATTACGGACAT3′ | 5′ATACCAGTGATTGCCATTC3′ |
MYL2 | chr12:111356969 | 5′TTGGAGACCTGAGTGTGGA3′ | 5′TGGGATTGTTTGGAGGATAG3′ |
MYH7 | chr14:23902749 | 5′GAAGGGACTCACTGGTAACTC3′ | 5′CTCTTTGGAGGGTCTGGA3′ |
TPM1 | chr15:63336266 | 5′CCTGTCTTTCCCTCTGTCTCT3′ | 5′AGTCCAATTTCAGCTGCTCTAT3′ |
TTN | chr2:179414991 | 5′ACCCCATAGTCCAAATTCTCA3′ | 5′AGTCTGCTGGTTTCCCGTT3′ |
TTN | chr2:179426798 | 5′GTGAACTGGAGAAAAGATGGTC3′ | 5′CTGGGGGACTGAATGGATA3′ |
TTN | chr2:179435192 | 5′CCTGTTCAATACTTTCACTGT3′ | 5′GCTGGTTTCTCTCCTTTC3′ |
TTN | chr2:179435225 | 5′CCTGTTCAATACTTTCACTGT3′ | 5′GCTGGTTTCTCTCCTTTC3′ |
TTN | chr2:179435281 | 5′CCTGTTCAATACTTTCACTGT3′ | 5′GCTGGTTTCTCTCCTTTC3′ |
TTN | chr2:179435636 | 5′CCTGTTCAATACTTTCACTGT3′ | 5′GCTGGTTTCTCTCCTTTC3′ |
TTN | chr2:179435664 | 5′CCTGTTCAATACTTTCACTGT3′ | 5′GCTGGTTTCTCTCCTTTC3′ |
TTN | chr2:179435678 | 5′CCTGTTCAATACTTTCACTGT3′ | 5′GCTGGTTTCTCTCCTTTC3′ |
TTN | chr2:179436004 | 5′CCTGTTCAATACTTTCACTGT3′ | 5′GCTGGTTTCTCTCCTTTC3′ |
TTN | chr2:179436043 | 5′CCTGTTCAATACTTTCACTGT3′ | 5′GCTGGTTTCTCTCCTTTC3′ |
TTN | chr2:179436062 | 5′CCTGTTCAATACTTTCACTGT3′ | 5′GCTGGTTTCTCTCCTTTC3′ |
TTN | chr2:179439808 | 5′AGCCAACAGAAACTACAGAGCC3′ | 5′GGTCCCAAGAGAAGGTTACAAA3′ |
TTN | chr2:179440715 | 5′GAGCATCAAAAAGTAGGAGAC3′ | 5′GTGGTGGCTGTGGAATAA3′ |
TTN | chr2:179442904 | 5′TTCACCAAAAAAGACAACAAC3′ | 5′AAACCAGTCAAACAAATACCAG3′ |
TTN | chr2: 179454374 | 5′GGTGGCTCAGAAATAACAAAC3′ | 5′TTAGATGAATCAGCACGGGT3′ |
TTN | chr2:179454375 | 5′GGTGGCTCAGAAATAACAAAC3′ | 5′TTAGATGAATCAGCACGGGT3′ |
TTN | chr2:179469995 | 5′GATGATGAAGGTGCGGAA3′ | 5′TTCTTAAACAGACACTGGATGC3′ |
TTN | chr2:179469996 | 5′GATGATGAAGGTGCGGAA3′ | 5′TTCTTAAACAGACACTGGATGC3′ |
TTN | chr2:179594978 | 5′CCCCCTTACTTTGTGGAA3′ | 5′AAAGTGAGGAGATGTAGAGACC3′ |
TTN | chr2:179596532 | 5′CTTGTGTTGCCAAAGTTATG3′ | 5′TCAGAAAAGCCAGTCCCT3′ |
TTN | chr2:179598095 | 5′TTCAGATTAGTTTTGGAGGC3′ | 5′AAAAGGTCAATATAGAAGAGTGC3′ |
TTN | chr2:179603055 | 5′TATTGTCTTCTTTTGCCTTCA3′ | 5′TGTTACTGTCTTGGTTGTTGG3′ |
TTN | chr2:179603066 | 5′TATTGTCTTCTTTTGCCTTCA3′ | 5′TGTTACTGTCTTGGTTGTTGG3′ |
TTN | chr2:179623776 | 5′AAAGACAAGAAAATCAAGCCA3′ | 5′CACAATGAAAAGTGGTAAAGGA3′ |
TTN | chr2:179643647 | 5′AGCGCATCAAACATGGAGAA3′ | 5′ACAGGGGCAAGAAATAAAAACT3′ |
MYOZ2 | chr4: 120072176 | 5′AGCATCCCCAGAGACATCA3′ | 5′AGTACCAGACTTCCCTACACAAT3′ |
MYOZ2 | chr4: 120072183 | 5′AGCATCCCCAGAGACATCA3′ | 5′AGTACCAGACTTCCCTACACAAT3′ |
PDLIM3 | chr4: 186429649 | 5′TTTTTCACCGTCTTCCCTTT3′ | 5′TTTGGTCCTTACCTGATTTCAT3′ |
PDLIM3 | chr4: 186429688 | 5′TTTTTCACCGTCTTCCCTTT3′ | 5′TTTGGTCCTTACCTGATTTCAT3′ |
LAMA4 | chr6: 112512883 | 5′ATTCCTCTTCAGATGTGCTC3′ | 5′TGTTGTGTTTGTGTCTCCTAGT3′ |
KIAA0196 | chr8: 126056890 | 5′ATTCCAAGTCAACACCCTA3′ | 5′CTTACAAAAACAGTCTAATCCTA3′ |