Fig. 8From: UPRmt activation improves pathological alterations in cellular models of mitochondrial diseasesMitochondrial protein expression and network in control and mutant GFM1 induced neurons (iNS). Image analysis of Mitotracker DeepRed FM and EF-GF1 (a)/mtCO2 (b) in control and mutant GFM1 iNS. Control and Mutant GFM1 iNS were incubated with Mitotracker DeepRed FM 100 nM for 45 min, then they were fixed and immunostained with anti-mtCO2 (mitochondrial encoded complex IV subunit) or EF-GF1 (mutated protein) and Tau (neuronal marker). Then, they were examined by fluorescence microscopy. Nuclei were revealed by Hoescht 1 μg/ml staining. iNs were treated with 1 µM tetracycline for 7 days. Scale bar = 15 μm. Fluorescence intensity quantification versus cell area shown in Additional file 1: Figure S9Back to article page