Fig. 7
From: UPRmt activation improves pathological alterations in cellular models of mitochondrial diseases
UPRmt proteins after ATF5 silencing. a Western blot analysis of the mutated protein (EF-G1) and several UPRmt-related proteins. ATF5 was silenced using a short hairpin RNA (shRNA) introduced by lentiviral particles against ATF5 RNA plus a puromycin selection marker. ATF5, ATF4, SIRT3 and Nrf1 were selected as UPRmt-related proteins, and mtCO2 as mitochondrial marker. b Band densitometry of a. c ATF4 expression levels after ATF4 silencing. A representative actin band is shown for all assays, although loading control was checked in every Western blot. d Band densitometry of Fig. 7c. Data represent the mean ± SD of 3 separate experiments. $p < 0.05 between untreated and treated control cells; *p < 0.05 between control and mutant GFM1 cells; ap < 0.05 Between non-silenced and silenced cells; A.U., arbitrary units. Puromycin selection was performed at 2 µg/ml concentration. KDa=Kilodalton, kDa