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Fig. 5 | Orphanet Journal of Rare Diseases

Fig. 5

From: UPRmt activation improves pathological alterations in cellular models of mitochondrial diseases

Fig. 5

Effect of tetracycline treatment on cell bioenergetics. a Mitochondrial respiration profile was measured with a Seahorse XFe24 analyzer. b Dipstick results of complex I activity in both colour (bluish-purple) and black/white. c Dipstick results of complex IV activity in both colour (yellowish) and black/white. Normalization was performed vs control. Control and mutant GFM1 fibroblasts were treated with 10 μM tetracycline for 7 days. Cells were seeded at a density of 1.5 × 104 cells/well with 500 µL growth medium (DMEM medium containing 10% FBS and 4,5 g/l glucose). 30 μg of protein were used for each dipstick assay.*p < 0.01 between Control and GFM1 fibroblasts. ap < 0.01 between untreated and treated mutant GFM1 fibroblasts. OCR, Oxygen Consumption Rate; a.u. (Arbitrary unit)

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