Fig. 4

Mitochondrial protein synthesis. a Cytosolic and mitochondrial protein synthesis. b Mitochondrial protein synthesis. Cycloheximide 50 μg/ml was added for 20 min to inhibit cytosolic protein synthesis previous cell staining. Cells were then treated with 10 μM tetracycline for seven days. Cell staining were performed by incubating with HPG 488 Alexa Fluor and Mitotracker DeepRed FM for 45 min. Then samples were fixed and nuclei stained with Hoescht 1 μg/ml. Red arrows represent positive colocalization with mitochondria and yellow arrows represent negative colocalization. R = Pearson Coefficient of correlation between Mitotracker and HPG. Scale bar in a 5 μm; Scale bar in b 3 μm. Chloramphenicol and chloramphenicol plus cycloheximide controls are shown in Additional file 1: Figure S6a and b respectively. Three coverslips per condition were analyzed. N = 20 cells. Full colocalization results are shown in Additional file 1: Figure S7