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Fig. 1 | Orphanet Journal of Rare Diseases

Fig. 1

From: UPRmt activation improves pathological alterations in cellular models of mitochondrial diseases

Fig. 1

Galactose medium screening and tetracycline treatment. Cells were initially seeded in glucose. After 3 days, glucose medium was changed to galactose. Images were acquired right after changing the medium to galactose (T0) and 72 h later (T72). In optimal conditions both cell lines present a similar proliferation rate (a, b, c, d). Control cells do not alter their growth rate (e, f) but mutant GFM1 cells undergo cell death after 72 h of culture in galactose medium (g, h). Tetracycline treatment does not affect control cells (i, j) but triggers the survival of mutant GFM1 cells in galactose medium. The proliferation rate of mutant GFM1 cells in galactose is slower than in normal conditions (k, l). Cells were cultured for 3 days in glucose, then the medium was changed to galactose and 10 µM tetracycline treatment was refreshed. Cell viability was assessed by live cell imaging counting and trypan blue 0.2% staining. Cell counting and representative images were acquired using the BioTek™ Cytation™ 1 Cell Imaging Multi-Mode Reader. Refer to Additional file 1: Figure S1 for the quantification of cellular proliferation. Scale bar = 40 μm

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