Fig. 6

HOTAIR silencing restrained RB growth in vivo. A Tumor volume was calculated by measuring length and width every 7 days after injection. B After 28 days, tumors were extracted from nude mice, and tumor weight was measured. C Ki67-positive cells were counted by IHC assay (the scale bar denotes 100 μm). D–E Expressions of Cleaved Caspase-3, Bcl-2, and phosphorylation levels of the PI3K/AKT pathway were determined by Western blot. N = 6. The results were presented as mean ± standard deviation and analyzed using the t test. **p < 0.01. The relative expressions of all proteins were normalized with GAPDH as the internal reference