Fig. 3

miR-20b-5p targeted RRM2. A Downstream target genes of miR-20b-5p were predicted on the ENCORI, RNAInter, Targetscan, and miRDB databases. B Coexpression relationship of target genes was searched on the Coexpedia database. C The binding site of miR-20b-5p and RRM2 was predicted on the ENCORI database. D The binding relationship between miR-20b-5p and RRM2 was verified using a dual-luciferase assay. E RRM2 level in normal retinal cells ARPE-19 and RB cells was detected using Western blot. F RRM2 level in RB cells Y79 and HXO-RB44 under different treatments was measured by Western blot. Cell experiment was repeated 3 times independently. The results were presented as mean ± standard deviation. Data in panels E and F were analyzed using the one-way ANOVA, and data in panel D were analyzed using the t test, followed by Tukey’s multiple comparisons test. **p < 0.01, ***p < 0.001. The relative expression of RRM2 was normalized to the internal reference GAPDH