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Fig. 2 | Orphanet Journal of Rare Diseases

Fig. 2

From: LncRNA HOTAIR facilitates proliferation and represses apoptosis of retinoblastoma cells through the miR-20b-5p/RRM2/PI3K/AKT axis

Fig. 2

HOTAIR regulated RB cell proliferation and apoptosis by serving as the ceRNA of miR-20b-5p. A Downstream miRNAs of lncRNA HOTAIR were predicted on the ENCORI and LncBase databases. B The binding site of HOTAIR and miR-20b-5p was predicted on the ENCORI database. C The binding relationship between HOTAIR and miR-20b-5p was verified using dual-luciferase assay. D miR-20b-5p expression in normal retinal cells ARPE-19 and RB cells was determined by qRT-PCR. E miR-20b-5p expression in RB cells under different treatments was determined by qRT-PCR. The combined treatment of HOTAIR silencing and miR-20b-5p downregulation using miR-20b-5p inhibitor was implemented on Y79 and HXO-RB44 cells. F Cell cycle was examined by flow cytometry. G Cell proliferative ability was assessed by CCK-8 assay. H Cell apoptosis was assessed by flow cytometry. I Expressions of Ki67, Cleaved Caspase-3, and Bcl-2 were determined by Western blot. Cell experiment was repeated 3 times independently. Data were presented as mean ± standard deviation. Data comparisons among multiple groups were analyzed using the one-way ANOVA, followed by Tukey’s multiple comparisons test, and pairwise comparisons were analyzed using t test. *p < 0.05, **p < 0.01, ***p < 0.001. The relative expression of miR-20b-5p was normalized to that in ARPE-19 cells with U6 as the internal reference; the relative expressions of Ki67, Cleaved Caspase-3, and Bcl-2 were normalized to the internal reference GAPDH

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