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Fig. 1 | Orphanet Journal of Rare Diseases

Fig. 1

From: LncRNA HOTAIR facilitates proliferation and represses apoptosis of retinoblastoma cells through the miR-20b-5p/RRM2/PI3K/AKT axis

Fig. 1

Downregulation of HOTAIR repressed RB cell proliferation and promoted apoptosis. Two types of normal retinal cells (ARPE-19 and RPE-1) and 4 types of RB cell lines (ORB50, Y79, HXO-RB44, and WERI-RB) were used. A HOTAIR expression was determined by qRT-PCR. After introducing sh-HOTAIR into Y79 and HXO-RB44 cells, B HOTAIR expression was determined by qRT-PCR. C Cell cycle was examined by flow cytometry; D cell proliferative ability was assessed by CCK-8 assay. E Cell apoptosis was assessed by flow cytometry. F Expressions of Ki67, Cleaved Caspase-3, and Bcl-2 were determined by Western blot. Cell experiment was repeated 3 times independently. The results were presented as mean ± standard deviation. Data in panel A were analyzed using the one-way ANOVA, and data in panels B–F were analyzed using the t test, followed by Tukey’s multiple comparisons test. In panel A, versus ARPE-19 or RPE-1; in panels B–F, versus corresponding control groups. **p < 0.01, ***p < 0.001. The relative expression of HOTAIR was normalized to that in ARPE-19 cells, with GAPDH as the internal reference; the relative expressions of Ki67, Cleaved Caspase-3, and Bcl-2 were normalized to the internal reference GAPDH

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