Fig. 3

In vitro sensitivity of blood T cells from a patient with homozygous variants in the NBN gene and heathy controls using the CDA treated with the indicated doses of A IR, B MMC, C ARA-C, and D Doxorubicin. The controls were assigned arbitrary numbers. Error bars indicate the standard deviation of technical replicates. E. PBMC from patients and controls were treated with two doses of IR and the γ-H2AX MFI in the treated sample was corrected for the background by subtracting the γ-H2AX MFI in the non-treated samples at 1 h and 24 h and plotted. Error bars indicate the standard deviation of the mean. Due to the lack of material, the analysis was carried out once for the patient, but 5 × 10³ and 15 × 10³ cells were analyzed at 1 and 24 h, respectively