Fig. 1

In vitro sensitivity of blood T cells from two siblings with variants in the PRKDC gene and two heathy controls using the CDA treated with the indicated doses of A IR or B MMC. The controls were assigned arbitrary numbers. Error bars indicate the standard deviation of technical replicates. C PBMC from patients and controls were treated with two doses of IR and the γ-H2AX MFI in the treated sample was corrected for the background by subtracting the γ-H2AX MFI in the non-treated samples at 24 h and plotted. Error bars indicate the standard deviation of the mean. The patient γ-H2AX MFI was compared with the controls using a two-tailed t test