Fig. 2

GO analysis of differentially expressed genes and functional exploration. a–c The PANTHER statistical over-representation test tool was used to determine over-representation of defined functional GO classes for the 1895 differentially expressed genes: a biological process; b molecular function; c cellular component. Expected and observed frequencies for each GO category are shown by gray and black bars respectively. The number of genes assigned to each category can be determined from the x-axis, and p value for each GO category is given on the right of each bar. d and e Non-redundant genes in over-represented GO categories were regrouped into functional classes and displayed as heatmaps: d GO categories related to transcription (GO:0010467; GO:0006396; GO:0016070; GO:0051252; GO:0003723; GO:0090304; GO:0019219; GO:0034654). e GO categories related to ribosome (GO:0022613; GO:0042254; GO:0016072; GO:0006364; GO:0030684; GO:0005730; GO:0030686; GO:1990904). f All mitochondrial differentially expressed genes, as determined by the BioMart R annotation package are displayed as a heatmap. g Expression of rRNA in LCL-AT and LCL-WT shown as a percentage of total RNA expression for 28S and 18S + 28S. h Analyze of nascent RNA transcription in LCL-AT and LCL-WT 1 h after 10 µM 5-ethynyl uridine (EU) incorporation. i Analyze of nascent protein synthesis in LCL-AT and LCL-WT 30 min after 50 µM O-Propargyl-puromycin (OPP) incorporation. h and i MFI: Mean fluorescence intensity. *pval < 0.05. Data are mean ± SD