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Fig. 1 | Orphanet Journal of Rare Diseases

Fig. 1

From: Gene expression analysis in EBV-infected ataxia-telangiectasia cell lines by RNA-sequencing reveals protein synthesis defect and immune abnormalities

Fig. 1

Overview of RNA-seq data generated from lymphoblastoid cell lines derived from 7 AT and 5 unaffected control individuals. a Experimental design and bioinformatic data analysis for dual RNA-seq. b Result of reads alignment on the human and EBV references genomes. For each sample, the number of mapped or unmapped read are shown on the y-axis. c Principal component analysis (PCA) plot visualizing the similarities between biological replicates and the separation between LCL-WT (red dots) and LCL-AT (blue dots). d Hierarchical clustering dendrogram of LCL-WT and LCL-AT normalized gene expression, with expression levels of differentially expressed genes with |log2FC|> 1 showed as a heatmap. Red represent downregulated genes and green represent upregulated genes. e Volcano plot displaying log2 fold change against − log10 (p-adjusted). DESeq2 analysis identified 1024 significant downregulated genes and 875 significant upregulated genes among which 414 had a log2 fold change < − 1 and 527 had a log2 fold change > 1

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Orphanet Journal of Rare Diseases

ISSN: 1750-1172