Fig. 2

Dose–effect of pantothenate treatment on PANK2 and mt-ACP expression levels. a Control (C1) and PKAN fibroblasts (P1, P2 and P3) were treated with increasing concentrations of pantothenate (1, 5, 10, 50, 500 μM) for 20 days. Protein extracts (50 μg) were separated on a SDS polyacrylamide gel and immunostained with antibodies against PANK2 and mt-ACP. b Densitometry of the Western blotting. Data represent the mean ± SD of three separate experiments. *p < 0.01 between PKAN patients and controls; ^ap < 0.01 between untreated and treated fibroblasts. A.U., arbitrary units