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Table 2 EXT1 and EXT2 primer sequences and PCR conditions

From: Mutation spectrum of EXT1 and EXT2 in the Saudi patients with hereditary multiple exostoses

Exons EXT1-Forward EXT1-Reverse Annealing
( ºC)
EXT2-Forward EXT2-Reverse Annealing
(ºC)
Exon 1a 5′ggaaaggcatccagagaaggt-3’ 5′-cttgcaaagggtgaaatcgaa-3’ 58 5′-cagtccgctccttcctttcct-3’ 5′-agtgcctggcccaacatgac-3 62
Exon 1b 5′-ttcgttccttgggatcaatt-3’ 5′-cctgtcctgggatgatcctta-3’ 56    
Exon 1c 5′-ggcacttggcctgactacac-3’ 5′-gggctcatccgccctcacc-3’ 58    
Exon 2 5′-gagttgctttgcgtaaattca-3’ 5′-acaccttctctttagctatcc-3’ 58 5′-aggttgaatagtcttttcaag-3’ 5′-ggaaaccaactcaagagcagaa-3’ 54
Exon 3 5′-cagtcattgagtttgtactga-3’ 5′-gagctgaccttttggattcat-3’ 58 5′-ggatccttgatagttgttgtc-3’ 5′-caattctgattacaaagtatg-3’ 58
Exon 4 5′-ctatatgctagaagccaaatg-3’ 5′-cactggaccaatcacacatcc-3’ 56 5′-gactcagtaattcctgttcct-3’ 5′-gcctcaaggaccctaccctg-3’ 56
Exon 5 5′-gtcactactctgactgccacc-3’ 5′-tgcagggtgttagatggacc-3’ 58 5′-ctggtaaggaaacacttactg-3’ 5′-ctagttgcatgctgaaaacaa-3’ 58
Exon 6 5′-ctccagcatgaggcagcggag-3’ 5′-gggtatgatgttagagaagt-3’ 58 5′-cagtattgcttggcgtcaacc-3’ 5′-tgtagtagttcttgaaccagg-3’ 58
Exon 7 5′-ctctttctgtctctgagaaga-3’ 5′-gaacagggagaagatatctag-3’ 58 5′-gatgttgtttctgcttgtgaa-3’ 5′-gatctagtggaggaagtaaac-3’ 56
Exon 8 5′-caggtgaggatgggagaattg-3’ 5′-gaagcattagcatcgtgcaac-3’ 58 5′-aaaggaattagcctaacctgg-3’ 5′-cctttacaattgtagtacatt-3’ 58
Exon 9 5′-gaattaatgtttcgccacagt-3’ 5′-ctgttaacaagatttggcctt-3’ 58 5′-caccaagcctgccatgtttgg-3’ 5′-ggtattgctattgacaaagca-3’ 58
Exon 10 5′-gacatgtttagggattcaaag-3’ 5′-ctcctcattatatgctcctgg-3’ 58 5′-gctgattctcccatctcattt-3’ 5′-ttacgcacaccttttggactc-3’ 58
Exon 11 5′-gctgcttgctcatttgcctg-3’ 5′-caggagttgagttctcattgg-3’ 58 5′-gatggtttgaacctaggaagt-3’ 5′-ctaagccctcttggcaggtat-3’ 58
Exon 12     5′-ccatgccttggctatgctgcc-3’ 5′-gttacaagaacttcctaggct-3’ 58
Exon 13     5′-caacatctcagcttacaacac-3’ 5′-tatggctaccagctgctgtcc-3’ 58
Exon 14     5′-ctctcaacctcttgaacatac-3’ 5′-gtgcatgccaagatccaagta-3’ 58
  1. PCR conditions: 50 ng of DNA were denatured at 95 °C for 5 min on initial cycle followed by 35 cycles of denaturation, annealing, and extension at 1 min on each step