Table 2 EXT1 and EXT2 primer sequences and PCR conditions
From: Mutation spectrum of EXT1 and EXT2 in the Saudi patients with hereditary multiple exostoses
Exons | EXT1-Forward | EXT1-Reverse | Annealing ( ºC) | EXT2-Forward | EXT2-Reverse | Annealing (ºC) |
|---|---|---|---|---|---|---|
Exon 1a | 5′ggaaaggcatccagagaaggt-3’ | 5′-cttgcaaagggtgaaatcgaa-3’ | 58 | 5′-cagtccgctccttcctttcct-3’ | 5′-agtgcctggcccaacatgac-3 | 62 |
Exon 1b | 5′-ttcgttccttgggatcaatt-3’ | 5′-cctgtcctgggatgatcctta-3’ | 56 |  |  |  |
Exon 1c | 5′-ggcacttggcctgactacac-3’ | 5′-gggctcatccgccctcacc-3’ | 58 |  |  |  |
Exon 2 | 5′-gagttgctttgcgtaaattca-3’ | 5′-acaccttctctttagctatcc-3’ | 58 | 5′-aggttgaatagtcttttcaag-3’ | 5′-ggaaaccaactcaagagcagaa-3’ | 54 |
Exon 3 | 5′-cagtcattgagtttgtactga-3’ | 5′-gagctgaccttttggattcat-3’ | 58 | 5′-ggatccttgatagttgttgtc-3’ | 5′-caattctgattacaaagtatg-3’ | 58 |
Exon 4 | 5′-ctatatgctagaagccaaatg-3’ | 5′-cactggaccaatcacacatcc-3’ | 56 | 5′-gactcagtaattcctgttcct-3’ | 5′-gcctcaaggaccctaccctg-3’ | 56 |
Exon 5 | 5′-gtcactactctgactgccacc-3’ | 5′-tgcagggtgttagatggacc-3’ | 58 | 5′-ctggtaaggaaacacttactg-3’ | 5′-ctagttgcatgctgaaaacaa-3’ | 58 |
Exon 6 | 5′-ctccagcatgaggcagcggag-3’ | 5′-gggtatgatgttagagaagt-3’ | 58 | 5′-cagtattgcttggcgtcaacc-3’ | 5′-tgtagtagttcttgaaccagg-3’ | 58 |
Exon 7 | 5′-ctctttctgtctctgagaaga-3’ | 5′-gaacagggagaagatatctag-3’ | 58 | 5′-gatgttgtttctgcttgtgaa-3’ | 5′-gatctagtggaggaagtaaac-3’ | 56 |
Exon 8 | 5′-caggtgaggatgggagaattg-3’ | 5′-gaagcattagcatcgtgcaac-3’ | 58 | 5′-aaaggaattagcctaacctgg-3’ | 5′-cctttacaattgtagtacatt-3’ | 58 |
Exon 9 | 5′-gaattaatgtttcgccacagt-3’ | 5′-ctgttaacaagatttggcctt-3’ | 58 | 5′-caccaagcctgccatgtttgg-3’ | 5′-ggtattgctattgacaaagca-3’ | 58 |
Exon 10 | 5′-gacatgtttagggattcaaag-3’ | 5′-ctcctcattatatgctcctgg-3’ | 58 | 5′-gctgattctcccatctcattt-3’ | 5′-ttacgcacaccttttggactc-3’ | 58 |
Exon 11 | 5′-gctgcttgctcatttgcctg-3’ | 5′-caggagttgagttctcattgg-3’ | 58 | 5′-gatggtttgaacctaggaagt-3’ | 5′-ctaagccctcttggcaggtat-3’ | 58 |
Exon 12 |  |  |  | 5′-ccatgccttggctatgctgcc-3’ | 5′-gttacaagaacttcctaggct-3’ | 58 |
Exon 13 |  |  |  | 5′-caacatctcagcttacaacac-3’ | 5′-tatggctaccagctgctgtcc-3’ | 58 |
Exon 14 |  |  |  | 5′-ctctcaacctcttgaacatac-3’ | 5′-gtgcatgccaagatccaagta-3’ | 58 |