Fig. 1From: Nationwide carrier detection and molecular characterization of β-thalassemia and hemoglobin E variants in Bangladeshi populationHRM curve analysis for mutation detection in β-globin gene targeting the hot-spot region. The temperature shifted difference curves generated by the mutant alleles of unknown samples could be easily distinguished from the wild type samples and identified by comparing to the controls with known mutations based on differences in the melting curve shapes. RFU, Relative Fluorescence Unit; * indicates novel mutationBack to article page