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Table 1 Optimized UHPLC conditions for the separation of oligosaccharide components

From: A new UHPLC-MS/MS method for the screening of urinary oligosaccharides expands the detection of storage disorders

Time (min)

A%

B%

Flow rate (ml/min)

0

5

95

0.5

5

60

40

0.5

13.30

60

40

0.5

13.50

30

70

0.5

15.50

30

70

0.5

17.50

5

95

0.5

27.50

5

95

0.5

  1. A: ammonium formate 5 mM + 0.05% (v/v) formic acid in water; B: ammonium formate 5 mM + 0.05% (v/v) formic acid in acetonitrile/water 90/10