Urinary extracellular vesicles (EVs)/mg creatinine from specific type of cells | Markers | Primary Hyperoxaluria Type 1 (PH1) | Statistical Results | |||
---|---|---|---|---|---|---|
PH-1 without NC or KS (n = 10) | PH-1 with NC (n = 6) | PH-1 with KS (n = 9) | p value | Q value | ||
Microvesicles/phosphatidylserine | Annexin-V | 14.4 (13.8, 15.2) | 13.4 (12.6, 13.9) | 12.3 (11.5, 13.6) | 0.13 | 0.16* |
Exosomes | CD63 | 14.0 (13.6, 14.4) | 13.1 (12.2, 14.1) | 12.8 (12.0, 13.4) | 0.14 | 0.16* |
Extracellular vesicles from glomerular cells | Â | Â | Â | Â | Â | Â |
Juxtaglomerular cells | Beta-1 adrenergic receptor | 14.7 (13.8, 14.8) | 13.8 (13.2, 14.1) | 12.8 (11.9, 13.8) | 0.10 | 0.16* |
Mesangial cells | SM22 alpha | 14.0 (12.7, 14.7) | 13 (12.5, 13.2) | 12.7 (11.9, 13.0) | 0.06 | 0.13* |
Podocytes | Nephrin | 13.7 (12.2, 14.3) | 13.7 (12.5, 14.6) | 12.0 (11.5, 12.7) | 0.03 | 0.12* |
Bowman’s capsule—parietal cells | Cytokeratin 8 | 13.9 (12.7, 14.7) | 13.2 (12.4, 14.0) | 12.2 (11.7, 13.5) | 0.26 | 0.28 |
Extracellular vesicles from different segments of nephron and renal pelvis | Â | Â | Â | Â | Â | Â |
Proximal tubule—simple cuboidal epithelium | Urate-anion Exchanger 1 | 12.8 (12.1, 13.9) | 11.9 (11.6, 14.2) | 11.4 (10.2, 12.0) | 0.01 | 0.11* |
Megalin | 14.2 (13.0, 14.6) | 12.9 (12.0, 14.4) | 11.9 (11.6, 13.0) | 0.04 | 0.12* | |
Thin loop of Henle – simple squamous epithelium | Urea Transporter (SLC14A2) | 11.9 (11.4, 12.8) | 11.3 (10.4, 13.3) | 11.1 (10.6, 12.4) | 0.64 | 0.64 |
Thick loop of Henle—simple cuboidal epithelium | Uromodulin | 15.0 (14.2, 15.7) | 14.1 (13.1, 15.0) | 12.6 (11.9, 14.4) | 0.05 | 0.12* |
Distal tubule—simple cuboidal epithelium | Prominin-2 | 14.5 (14.0, 15.2) | 13.8 (12.7, 14.5) | 13.0 (12.7, 13.2) | 0.03 | 0.12* |
Collecting duct—principal cells | Aquaporin-2 | 14.5 (13.7, 14.8) | 13.8 (13.1, 14.4) | 12.6 (12.0, 13.6) | 0.09 | 0.16* |
Renal pelvis—transitional epithelium | Cytokeratin 20 | 13.9 (12.3, 14.9) | 12.8 (11.4, 14.5) | 11.6 (11.5, 13.4) | 0.12 | 0.16* |