Fig. 3
From: Novel GANAB variants associated with polycystic liver disease
Protein analysis of wild type and mutant GIIα in HEK293 cells. Western blot detection using anti-Flag antibody of cell lysate from transiently transfected HEK293 cells using N-terminal Flag tagged constructs with: a GANAB Wild Type (WT) and variants c.11_16delTAGCGG (11), c.687delT (687) and c.2002+1G>C (INT) at te left of the marker (M). The constructs showed similar amounts of expressed GIIα with predicted molecular weights; b stable transfected HEK293 cells resulted in reduced level of truncated GIIα (INT) compared to the normal GIIα (WT) using the intron construct