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Table 1 Oligonucleotides used to carry out the site directed mutagenesis in the human F12 cDNA that remove the two N-glycosylation sites

From: Factor XII in PMM2-CDG patients: role of N-glycosylation in the secretion and function of the first element of the contact pathway

Mutation

Oligonucleotide sequence (5′–3′)

Asn230Lys

Forward: CACCTACCGGAAAGTGACTGCCGAG

Reverse: CTCGGCAGTCACTTTCCGGTAGGTG

Asn414Lys

Forward: CAGGAACGCCGTAAACACAGCTGTGAGC

Reverse: GCTCACAGCTGTGTTTACGGCGTTCCG

Thr309Lys

Forward: GAAGCCTCAGCCCAAGACCCGGACCCCGC

Reverse: GCGGGGTCCGGGTCTTGGGCTGAGGTTC

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Orphanet Journal of Rare Diseases

ISSN: 1750-1172