Fig. 4

Apoptosis and ROS production of patient and control PMN. a Percentage of apoptotic PMN (Annexin V positive) after 0, 6 and 12 h culture showing equal rates of apoptotic cells for healthy donors and patient. b percentage of apoptotic PMN (Annexin V positive) in healthy donors and patient after 6 h culture in the presence of 100 ng/ml GM-CSF. c Fraction of apoptotic cells (Annexin V positive) collected from healthy donors and patient after 12 h of caspase inhibition with 1 μM Q-VD. d ROS production in patient and healthy donor PMN measured by rhodamine fluorescence. Fluorescence intensity of DHR123-loaded PMN in basal conditions (PBS) or after activation by PMA