Fig. 4
From: Synergistic apoptosis following endoplasmic reticulum stress aggravation in mucinous colon cancer
Combination of celecoxib and orlistat reduced mucinous tumor growth in vivo. Murine xenograft models of intraperitoneal (IP) luciferase-labelled LS174T cells were treated with PBS alone (control), or celecoxib (C) alone (200 mg/kg b.w.), or orlistat (O) alone (10 mg/kg b.w.), or combination of C + O every other day (starting on day 7 following tumor implantation) until they were euthanized at day 28. Gross intra-abdominal tumor burden is depicted pictorially on day 28; serial weekly changes in mean luciferase intensity during treatment for 3 weeks are shown a; error bars represent standard error of the mean among the 6 xenografts in each treatment group. Harvested tumor tissue from euthanized mice (day 28) was subjected to TUNEL IF assay b and IF for ERS markers c; the bar graphs demonstrate mean intensity difference. Asterisk represents a statistically significant difference compared with the control group (* p < 0.05, ** p < 0.01)