Fig. 1
From: Synergistic apoptosis following endoplasmic reticulum stress aggravation in mucinous colon cancer
Mucinous colon cancers and high MUC2 producing cell lines exhibit elevated basal ERS that correlates with MUC2 expression levels. a Representative pictures from immunofluorescence (IF) analysis of human explant tissue comparing MUC2 and ERS proteins (GRP78 [BiP], ATF4, CHOP) between 5 mucinous colon cancer (MCC) and 5 non-MCC (NMCC); the bar graphs demonstrate mean intensity difference. b Representative pictures from IF analysis of MUC2 and ERS proteins (GRP78 [BiP], ATF4, CHOP) comparing high-MUC2 expressing cells (dnTCF4-LS174T cells exposed to doxycycline for 48 h) versus low-MUC2 expressing cells (dnTCF4-LS174T cells without doxycycline exposure); the bar graphs demonstrate mean intensity difference. c Representative pictures from IF analysis of MUC2 and GRP78 (BiP) comparing stable MUC2 knockdown (KD) in LS174T cells compared to lentiviral (LV) control cells; western blot shows MUC2 protein expression in MUC2 KD cells compared to lentiviral control cells. Error bars represents standard deviation (S.D.) from triplicate experiments (*** p < 0.001)