Fig. 2

Electron and immune-electron microscopy of the gastric biopsy. a Muscularis mucosae showing typical osmiophilic intracytoplasmic bodies (arrows); b Vascular smooth muscle cell with GB3 accumulation (arrows); c Epithelial cell with multiple osmiophilic intracytoplasmic, lamellar bodies; d High magnification view demonstrating the typical parallel stacked lamellae with a zebra-like pattern that characterizes the GB3 bodies; the red-squared figure (upper right corner) shows the original body at low magnification. e Immunoelectron micrograph demonstrating the specific reaction of anti-GB3 antibodies in the osmiophilic bodies’ accumulations; f Epithelial cells of the gastric mucosae showing intra-cytoplasmic accumulation of GB3 specifically immunostained with anti-GB3 antibodies; g The red-squared area in (f) is shown at higher magnification in (g); h Immunoelectron micrograph demonstrating the specific reaction of GB3 antibodies in a non-vascular SMC, a fine extension of the muscularis mucosae extending up between the glands. The red-squared figure (upper right corner) shows the original cell at low magnification