Fig. 3
From: CLN5 in heterozygosis may protect against the development of tumors in a VHL patient
a Cell count by Bio-Rad TC20 Automated Cell Counter. Primary HB cells were trypsinized 72 h after transfection with siCLN5 and siScl as negative control. Cell viability was measured in 786-O cells (b) and ccRCC primary cells (c) 48 h after transduction by a luciferase assay, where ATP levels are a direct correlation of cell viability. d ccRCC primary cell cultures under the microscope were photographed 72 h after transfection. Control group was left untreated, while siScl serves as negative control. Every condition was replicated three times – the graphs show the combined mean of these experiments, representing the obtained standard deviation in the error bars. Statistical significance is represented as *p < 0.05; **p < 0.01; and ***p < 0.001