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Fig. 3 | Orphanet Journal of Rare Diseases

Fig. 3

From: Prophylactic treatment of rapamycin ameliorates naturally developing and episode -induced heterotopic ossification in mice expressing human mutant ACVR1

Fig. 3

Rapamycin inhibits pinch-injury-induced HO in FOP-ACVR1 mice. a Schematic view of the experiments. indicates the day of the pinch-injury. b Representative μCT images and histological findings of the injured sites of mice in the control and injury-initiated groups. Tissue sections were stained with H&E, Safranin O, or von Kossa. Scale bar = 1000 μm. c Quantification of HO at the injured sites. The HO volume was measured from the μCT images of mice in the control (n = 5), injury-initiated (n = 6), and early treatment (n = 6) groups. *, p < 0.05, Student’s t-test. d Representative μCT images of HO in the contralateral limbs of mice in each group. Arrows indicate HO. e Quantification of HO in the contralateral limbs. The HO volume was measured from the μCT images of mice in the control (n = 5), injury-initiated (n = 6), and early treatment (n = 6) groups. *, p < 0.05, Student’s t-test

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