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Fig. 2 | Orphanet Journal of Rare Diseases

Fig. 2

From: Prophylactic treatment of rapamycin ameliorates naturally developing and episode -induced heterotopic ossification in mice expressing human mutant ACVR1

Fig. 2

Pinch-injury induces HO in FOP-ACVR1 mice. a Histological findings of the injured sites. Samples were taken from the injured sites of DOX (−) or DOX (+) mice at each time point and analyzed by Hematoxylin-Eosin (H&E), Safranin O, and von Kossa staining. Scale bars = 200 μm. b Representative soft X-ray images of the injured limbs of DOX (−) or DOX (+) mice at each time point. c Schematic view of the experiments. indicates the day of the pinch-injury. d mRNA expression levels of cytokines in spleen cells at Day 28 in c. The expression levels in each group are relative to those of the DOX (−)/Pinch (−) group. n = 3 for all groups except the DOX (+)/Pinch (+) group (n = 4). *, p < 0.05, Student’s t-test

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