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Table 1 Animal experiments: overview, set-up and analysis details

From: Frizzled related protein deficiency impairs muscle strength, gait and calpain 3 levels

Experiment ID Experiment details
1. General and weight analysis * 5 to 8-week-old male and female C57Bl/6 J and Frzb−/− mice
* Primary outcome: 5-week-old mice body weight, Fig. 1a
* Total sample size: n = 29; WT C57Bl/6 J: n = 15 and Frzb−/−: n = 14
* Secondary outcome: 8-week-old mice body weight, Fig. 1b
* Total sample size: n = 31; WT C57Bl/6 J: n = 20 and Frzb−/−: n = 11
2. Muscle analysis * 5 to 6-week-old male and female C57Bl/6 J and Frzb−/− mice
* Primary outcome: Mice muscles’ weight, Fig. 1c
* Total sample size: n = 30; WT C57Bl/6 J: n = 16 and Frzb−/−: n = 14
* Secondary outcome: Myosin heavy chain composition, Fig. 1d
* Total sample size: WT C57Bl/6 J: n = 3–2 and Frzb−/−: n = 3–4
* Secondary outcome: Hanging time, Fig. 1e
* Total sample size: n = 16; WT C57Bl/6 J: n = 7 and Frzb−/−: n = 9
3. Catwalk analysis * 8-week-old male and female C57Bl/6 J and Frzb−/− mice
* Total sample size (8-week-old): n = 36; WT: n = 20, Frzb−/−: n = 16
* Primary outcome: Stand, Fig. 2a
* Secondary outcome: Swing phase, Fig. 2b
* Secondary outcome: Step cycle, Fig. 2c
* Secondary outcome: Stride length, Fig. 2d
4. Chronic exercise protocol * 5-week-old male and female C57Bl/6 J and Frzb−/− mice
* Total sample size: n = 16; WT: n = 9, Frzb−/−: n = 7
* Primary outcome: Mice body weight, Fig. 3a
* Secondary outcome: Soleus CSA and histology, Fig. 3b-c
* Secondary outcome: Tibialis anterior fibre type composition, Fig. 3d
5. Cardiotoxin injection * 10-week-old male and female C57Bl/6 J and Frzb−/− mice
* Total sample size: n = 39; WT: n = 19, Frzb−/−: n = 20
* Primary outcome: Hematoxylin and eosin stained Tibialis anterior, Fig. 4a
* Secondary outcome: Hematoxylin and eosin stained Soleus, Fig. 4b
6. Satellite cell isolation * 4-week-old male and female C57Bl/6 J and Frzb−/− mice
* Total sample size: n = 14; WT: n = 7, Frzb−/−: n = 7
* Primary outcome: Satellite cells and myotubes immunofluorescence analysis, Fig. 5