Fig. 5From: Frizzled related protein deficiency impairs muscle strength, gait and calpain 3 levelsFrzb−/− mice have increased MyoD and Ki67 positive satellite cells but no detectable differences in differentiation capacity. a Immunofluorescence analysis of satellite cells extracted from 4-week-old WT and Frzb−/−. Left images stained for MyoD (green) likewise right images for Ki67 (red). Percentage of MyoD and Ki67 positive nuclei [n = 7 (WT 3 male and 4 female) and 6 (Frzb−/− 3 male and 3 female), P = 0.0046 and P < 0.0001 respectively, t-test]. b Immunofluorescence analysis of WT and Frzb−/− myotubes at day 3 of differentiation. Left images stained for nuclear myogenin (red) and cytoplasmatic sarcomeric α-actinin (green). Right images stained for nuclear MyoD (green) and cytoplasmatic MyHC (red). Myotubes fusion index, calculated as the percentage of nuclei inside myotubes on the total amount of nuclei. In all cases nuclei were visualized with Hoechst (blue). Scale bar 250 μm. Error bars indicate mean ± SEMBack to article page