Fig. 3

Frzb^−/− and wild-type (WT) mice do not respond differently to exercise. a Differences between WT and Frzb^−/− body weight during treadmill exercise [n = 4 (WT-not-trained male), 5 (WT-trained male), 2 (Frzb^−/−-not-trained male and female) and 3 (Frzb^−/−-Trained male), p < 0.001 for Frzb^−/− vs WT over time by two-way ANOVA]. b Hematoxylin-eosin staining of Soleus from WT and Frzb^−/− mice at the end of the treadmill experiment (scale bar 250 μm) and (c) muscle fibre cross-sectional area measurement [n = 4 (WT-not-trained male), 5 (WT-trained male), 3 (Frzb^−/−-not-trained 1 male and 3 female) and 3 (Frzb^−/−-Trained male)] * p = 0.0066 for genotype by two-way ANOVA]. d SDH staining of Tibialis anterior from WT and Frzb^−/− at the end of the experiment and fibre type quantification. e Real-time PCR analysis of Fbx32, Murf1, Myh3, Myh2, Pax7, Myod and Myog in Gastrocnemius of WT and Frzb^−/− mice (n = 3 male for each group). Error bars indicate mean ± SEM