Fig. 1

Frzb^−/− mice are smaller than wild-types (WT) with reduced muscle size and strength. a-b Body weight (g) of 5- and 8-week-old WT and Frzb^−/− mice [n = 8 WT-females, 7 WT-males, 6 Frzb^−/−-females and 8 Frzb^−/−-males; *** p < 0.0001, p < 0.0001 and ^**p = 0.01, Bonferroni-corrected for two tests in two-way ANOVA]. c Muscle weights (mg) of 4–5-week-old WT and Frzb^−/− mice [n = 15 WT and 14 Frzb^−/− mice, ^*** p < 0.0001, **p = 0.009, Bonferroni-corrected for four tests in two-way ANOVA]. d Real-time PCR analysis of Myh1, Myh2 and Myh4 in Tibialis anterior and Soleus of WT and Frzb^−/− mice (n = 3 WT and 3 Frzb^−/− mice for Tibialis anterior and 2 WT and 4 Frzb^−/− for Soleus samples). e Muscle strength reflected by hanging time in male WT and Frzb^−/− mice [n = 9 WT and 7 Frzb^−/− mice, * p = 0.048 for effect of genotype, p = 0.8 for effect of weight in two-way ANOVA]. Error bars indicate mean ± SEM