Skip to main content

Advertisement

Springer Nature is making SARS-CoV-2 and COVID-19 research free. View research | View latest news | Sign up for updates

Fig. 5 | Orphanet Journal of Rare Diseases

Fig. 5

From: Comparison of zebrafish and mice knockouts for Megalencephalic Leukoencephalopathy proteins indicates that GlialCAM/MLC1 forms a functional unit

Fig. 5

Mlc1 is mislocalized in primary Glialcam−/− astrocytes. Localization of GlialCAM (a) and Mlc1 (b) in primary astrocytes from wild-type (WT, left), Glialcam−/− (middle) and Glialcam−/− complemented with adenoviruses expressing human GlialCAM (right). In WT and complemented astrocytes, GlialCAM and Mlc1 are located at cell-cell junctions (arrowheads). Scale bar: 10 μm. (c) GlialCAM and Mlc1 protein levels primary astrocytes from wild-type (WT, left), Glialcam−/− (middle) and Glialcam−/− complemented with an adenoviruss expressing human GlialCAM. Actin served as a loading control. Two other independent experiments gave similar results. Densitometric analysis (n = 3) indicates that Mlc1 levels were reduced in astrocytes from Glialcam−/− mice and expression was recovered after expression of GlialCAM using adenoviruses. * p < 0.05 vs wild-type astrocytes

Back to article page