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Fig. 4 | Orphanet Journal of Rare Diseases

Fig. 4

From: Comparison of zebrafish and mice knockouts for Megalencephalic Leukoencephalopathy proteins indicates that GlialCAM/MLC1 forms a functional unit

Fig. 4

mlc1 expression and localization in glialcama−/− zebrafish. a Quantitative real-time PCR to determine levels of glialcama, glialcamb and mlc1 messenger RNA in the brain of glialcama−/− zebrafish. Bars, relative expression levels compared with WT sibling; error bars, s.e. (n ≥ 3). **P < 0.01 (vs. wild-type, two-way ANOVA) b Comparison of mlc1 protein levels in brain of wild-type (WT), mlc1−/−, glialcama−/− and mlc1glialcama−/− zebrafish by Western blots of extracts from 5-months-old zebrafish. Western blot is representative of three independent experiments. Tubulin served as a loading control. c, d mlc1 expression (arrowheads) observed in the optic tract of both wild type (c) and glialcama−/− (d) brains. e, f mlc1 expression is restricted to the inner limiting membrane of the retina (arrowheads) both in wild type (e) and glialcama−/− (f). Scale bars: 50 μm (c, d); 60 μm (e, f)

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