Fig. 3

Myelin vacuolization in Glialcam^−/−, Mlc1^−/− and Glialcam^−/−Mlc1^−/− mouse models. Haematoxylin-eosin staining of sagittal sections of the cerebellum of 19- and 61-week-old mice showed similar levels of myelin vacuolization in Glialcam^−/−, Mlc1^−/− and Glialcam^−/−Mlc1^−/− animals. As a control we show the same area of a wild-type mouse at 19 weeks. The inset shows the percentage of vacuolation in double KO animal versus the vacuolation observed in Glialcam KO animals (n = 3) and Mlc1 KO animals (n = 3) considering each age independently, without substracting the minor vacuolization observed in wild-type animals. Data were analyzed by GraphPad Prism software. In order to compare the different groups (dKO vs Glialcam^−/− and dKO vs Mlc1^−/−), one-way analysis of variance (ANOVA) followed by post-hoc Bonferroni’s multiple comparison test was used. ns: not significative. Scale bar, 400 μm