Fig. 2

Smooth muscle cell (SMC) distribution and Ki67 expression in CPAM and in control lung tissue. Immunostaining of CPAM 1, CPAM 2 and control lung with ACTA2 and Ki67 antibodies. a. CPAM 1, CPAM 2 and adjacent control lung sections co-stained with anti-ACTA2 (purple) and anti-Ki67 (dark brown, black arrows in the magnified area at the right panel). b. Comparison of the maximal thicknesses of ACTA2 staining in bronchi, CPAM 1 and CPAM 2 in microns. CPAM 1 compared to CPAM 2 sample: ** p < 0.01. Bronchi compared to CPAM 2 sample: ♦♦ p < 0.01. c. Graph comparing the ratio of ACTA2 stained surface to the total measured surface. CPAM 1 compared to CPAM 2: ** p < 0.05. N = 3 different patients, each point corresponds to the mean of 10 random pictures per sample. d. Graph comparing the percentages of Ki67 epithelial positive cells between groups. N = 3 different patients, each point corresponds to the mean of 10 random pictures per sample. Scale bars: 100 μm