Fig. 4From: Arabidopsis thaliana alternative dehydrogenases: a potential therapy for mitochondrial complex I deficiency? Perspectives and pitfallsEvaluation of mitochondrial respiration: Mitochondrial membrane potential variations assessed by rhodamine 123 fluorescence and oxygen uptake measured with optode device in digitonine permeabilized fibroblasts (representative graphs for the control fibroblasts - left panel and AtNDA2-transfected control fibroblasts - right panel). The reaction was started by the addition of glutamate/malate, followed by injections of ADP (see text). Note that the amount of oxygen reduced during ADP phosphorylation is significantly higher in AtNDA2 transfected cells comparing to controlBack to article page