Fig. 5

MMP levels are increased in the lungs of HPS1 mice. a Transcript levels for Mmp-2, − 3, − 8, − 9, − 12, − 13 and − 14 in age-matched control and HPS1 lungs at baseline (n = 4 each group, p < 0.05 vs control). b Western blot (left) for MMP-2 and MMP-9 in whole lung digests from control and HPS1 mice at baseline. c Gelatin zymography for MMP-2 and MMP-9 in lungs of control and HPS1 mice (top). Activity of MMP-2 but not MMP-9 was significantly increased in the lungs of HPS1 mice. d Transcript levels for Mmp-2, − 3,-7, − 8, − 9, − 12, − 13 and − 14 in age-matched control and HPS1 lungs 7 days after bleomycin (n = 4 each group, p < 0.05 vs control). e Western blot for MMP-2 and MMP-9 in whole lung digests from control and HPS1 mice at 7 days after bleomycin. Immunoblots are representative of at least two different blots and densitometry analyses (bar graphs) are representative of n = 5 or more mouse specimens (*p < 0.05, HPS2 vs control). Data are expressed as mean ± SE, and statistical significance was assessed using a Student’s unpaired t test or multiple comparisons