Fig. 5

Control MG and OP-MG myocytes show different gene expression profiles. RNA was extracted from untreated and MG sera (MGS) treated control MG (n = 6) and OP-MG (n = 10) myocytes after 48 h differentiation as described. Target gene expression levels were determined using the custom qPCR gene expression array and a fold change in gene expression (OP-MG/control MG) was calculated for each gene in the early untreated and MGS treated models. Genes with statistically significant (p < 0.02) fold changes are shown and the remaining genes (0.02 < p < 0.05) are shown in Additional file 1: Figure S2. a. shows the fold change as an average of OP-MG/control MG samples (error bars show mean and 95% CI) and b. shows the 2^-∆Cq values for each sample (open circles = control MG, closed circles = OP-MG). Student’s t test was used for comparisons where the data was normally distributed, otherwise Mann-Whitney test was used (†) where Shapiro-Wilk normality test p < 0.05