Molecular and cellular characterizations of human cherubism: disease aggressiveness depends on osteoclast differentiation

Table 3 Identification of cherubism granuloma cells (CD5, CD3, CD4, CD8, CD20, CD68, AE1/AE3, Vimentin, TRAP activity)

	Cells/20HPF
	CD5 Lymphocytes	CD20 B-Cells	CD3 T-Cells	CD4 LT4	CD8 CD8+	CD68 mononuclear Monocytes	CD68 Multinuclear Osteoclast-like	Vimentin Semi-quantitative	AE1/AE3	TRAP Osteoclast-like
1-A	15 [9–20]	0	14.8 [8–18]	0	14.6 [8–21]	5.4 [3–9]	1.4 [0–3]	+++	0	0
1-B1	8 [6–9]	0.2 [0–1]	7 [4–9]	0.6 [0–2]	6.8 [2–9]	18 [9–32]	3.2 [1–5]	+++	0	4 [0–6]
1-B2	2.6 [0–7]	0	1.6 [0–3]	0.6 [0–1]	2 [1–3]	34.24 [31–37]	2.8 [1–5]	+++	0	0
1-C1	19.2 [8–37]	2.4 [0–6]	29.2 [18–30]	4.4 [0–11]	21.6 [6–32]	14 [6–22]	8,2[0–10]	+++	0	8.6 [8–10]
1-C2	31 [15–48]	0.2 [0–1]	19.6 [15–27]	4.2 [0–10]	17 [4–37)	21.8 [10–35]	13.2 [7–20]	+++	0	7.4 [3–11]
2-A	0.6 [0–2]	0	0	0	0	0.8 [0–2]	0.4 [0–2]	+++	0	0
2-B	21.4 [10–33]	0.6 [0–2]	31.6 [27–35]	0.6 [0–2]	7.2 [3–15]	14.6 [8–19]	2.8 [2–6]	+++	0	3.2 [1–5]

Number of positive cells was evaluated for each assay from five randomly selected areas (200× high-power field; HPF). Vimentin expression was semi-quantitatively evaluated (0 = vimentin negative; + few positive cells; ++ less than the half positive cells; +++ more than half positive cells). CD5 identifies lymphoid cells; CD3, T cells; CD4, T4 cells; CD8, CD8 + cells; CD20, B cells; CD68 determines monocyte lineage cells, immature macrophages (mononucleated cells), macrophages or osteoclasts (multinucleated cells); AE1/AE3 identifies epithelial cells, vimentin mesenchymal cells, TRAP activity in osteoclast cells when multinucleated (> 3 nuclei)

ISSN: 1750-1172