Fig. 5

Cherubism MGC are osteoclasts. a-d: TRAP Assay, × 20 (scale bar = 50 μm), E and F: Resorption assay × 10 (scale bar = 200 μm). a-c, Case 2-B. Cherubism cells cultured in standard (a) and osteoclastogenic (c) medium at day 3 showed that MGC are TRAP-positive, b-d Case 2-B. Cherubism cells cultured in standard (b) and osteoclastogenic (d) medium at day 7. Osteoclast number, osteoclast size and nuclei number increased from day 3 to day 7. e-f Case 2-B. Bone slices displayed pit formation at day 7 in standard (e) osteoclastogenic medium (f) (arrow). These images are representative of what was observed for all the cultures